Passive transfer of the monoclonal antibody (MAb) particular for glycoprotein D (gD) is certainly impressive in avoiding the development of herpes simplex virus type 1-induced stromal keratitis. desired cell populace and infected around the scarified cornea with herpes simplex virus type 1 uniformly developed necrotizing stromal keratitis by 3 weeks postinfection. purchase Saracatinib A single inoculation of anti-gD MAb (55 micrograms) given intraperitoneally 24 h postinfection strongly guarded hosts depleted of CD4+ cells against stromal keratitis. Similarly, antibody treatment in CD8+ or asialo GM1+ cell-depleted hosts was as therapeutically effective as that seen in non-cell-depleted purchase Saracatinib mice. We also observed that in cell-depleted mice, the virus spread into the central nervous system and caused encephalitis. The CD4+ cell-depleted mice were the most severely affected, as 100% developed fatal disease. Anti-gD MAb treatment successfully guarded all (32 of 32) CD4+-, CD8+-, or asialo GM1(+)-depleted hosts against encephalitis. We therefore conclude that antibody-mediated prevention of stromal keratitis and encephalitis does not require the purchase Saracatinib obligatory participation of CD4+, CD8+, or asialo GM1+ cells. However, when mice were simultaneously depleted of both CD4+ and CD8+ T-cell subsets, antibody treatment could not prevent fatal encephalitis. Thus, antibody can compensate for the functional loss of one but not two T-lymphocyte subpopulations. Full text Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (1.3M), or click on a page image below to purchase Saracatinib browse page by page. Links to PubMed are also available for Selected Recommendations.? 6008 Rabbit Polyclonal to HRH2 6009 6010 6011 6012 6013 6014 ? Selected.