Relative gene expression was normalized to GAPDH and calculated according to the CT method for qPCR

Relative gene expression was normalized to GAPDH and calculated according to the CT method for qPCR. Metabolic Assay ECAR measurements were done as described previously (Zhou et?al., 2012) with an optical fluorescent oxygen/hydrogen sensor XFe96 Seahorse analyzer. the primed state. Forced expression of active RAS and RAS inhibition have shown that RAS regulates glycolysis, CADHERIN expression, and the expression of repressive epigenetic marks in pluripotent stem cells. Altogether, this study indicates that RAS is located at a key junction of early ESC differentiation controlling key processes in priming of naive cells. and in a teratoma assay, only naive PSCs are able to efficiently contribute to the formation of chimeric animals (Rossant, 2008). Naive state culture of murine embryonic stem cells (mESCs) can be sustained in the presence of serum and leukemia inhibitory factor (fetal calf serum [FCS]/LIF). However, a more uniform ground state culture that mirrors better the undifferentiated transcriptional and epigenetic landscape of pre-implantation epiblast cells can be achieved in the presence of a combination of LIF and the inhibitors of MEK and GSK (2i/LIF) (Hackett and Azim Surani, 2014, Nichols and Smith, 2009, Weinberger et?al., 2016, Wray et?al., 2010, Ying et?al., 2008). In contrast to mouse PSCs (mPSCs) that display features of naive state, human PSCs (hPSCs) are believed to be stabilized in a primed state of pluripotency. Cells that are at naive state are considered to be more amenable for genetic manipulation, and are able to differentiate more uniformly. Thus, many efforts have been made to characterize the molecular pathways regulating pluripotency states (Boroviak et?al., 2014, Buecker et?al., 2014, Guo et?al., 2009, Hackett and Azim Surani, 2014, Kalkan and Smith, 2014, Weinberger et?al., 2016), and in particular to convert primed hPSCs into naive state (Chan et?al., 2013, Gafni et?al., 2013, Takashima et?al., 2015, Theunissen et?al., 2014, Ware et?al., 2014, Yang et?al., CPPHA 2017). Yet, there is a controversy regarding the quality of the resulting cells, to what extent they appropriately reflect preimplantation cells, while culture conditions typically require the combination of multiple soluble factors and inhibitors. Therefore, a better understanding of the signaling pathways that control self-renewal at the different states of pluripotency is necessary. Optimized culture of naive cells would allow an appropriate study of early development and CPPHA lineage commitments using PSCs and their efficient application. GSS Interestingly, the transition from naive to primed state is accompanied by cellular changes that are to some extent similar to cancer cell transformation. These changes include metabolic switch from oxidative phosphorylation to anaerobic glycolysis, marks of epithelial-mesenchymal transition (EMT), and drastic epigenetic changes, suggesting that this process may be mediated by oncogenic pathways. The role of RAS proteins has been extensively studied in the field of cancer cell biology; however, their involvement in stem cells and CPPHA cellular reprogramming remained largely unexplored. The three RAS isoforms, namely, H-RAS, K-RAS, and N-RAS, are encoded by three separate genes and they possess many overlapping roles, although some isoform-specific features has been reported (Prior and Hancock, 2012, Schubbert et?al., 2007). RAS proteins act as molecular switches, alternating between inactive guanosine diphosphate (GDP)-bound state and active guanosine triphosphate (GTP)-bound state. Upon receptor-mediated signal transduction, RAS proteins become active (GTP bound) and undergo allosteric change in their conformation, allowing them to recruit a large set of proteins known as Ras effector proteins (Mitin et?al., 2005, Vigil et?al., 2010). Among these are mitogen-activated?protein kinase and phosphatidylinositol 3-kinase (PI3K), which regulate a cascade of signals leading to a wide range of cellular responses, including growth, differentiation, inflammation, survival, and apoptosis. Although RAS proteins are involved in many biological processes in health and disease, their involvement in early embryogenesis and ESC differentiation remained largely unexplored. Here, we show that all three RAS isoforms are activated upon early ESC differentiation. While low RAS activity hallmarks the naive state of pluripotency, RAS activation is necessary and sufficient to CPPHA induce key features of differentiation, indicating that RAS is located at a key junction of this process. Inhibition of RAS significantly attenuates differentiation, while its ectopic expression.

Comments are closed.