Background: Today’s work aimed to investigate the manifestation of CD160/ CD200 in CLL and additional mature B-cell neoplasms (MBN) and their use as an additional diagnostic tool for differentiating CLL from additional MBN. B-NHL. However, double negative manifestation of both markers was found only in 24% of the B-NHL individuals. Conclusion CHMFL-ABL/KIT-155 : CD160 with CD200 can be used as additional diagnostic markers to the available routine panel to differentiate between B-CLL and additional non-specified B-NHL individuals. Key Terms: CLL, Mature B-cell neoplasms, CD200, CD160, Flow cytometry Intro B-cell chronic lymphoproliferative disorders (B-CLPD) include a heterogeneous group of disease entities arising from clonal proliferation of adult B-lymphocytes1. B-CLPD is now most often diagnosed by circulation cytometric immunophenotyping that identifies a clonal light-chain restricted human population expressing B-cell markers in the blood or BM 2. However, a final analysis cannot be carried out in all individuals with B-CLPD by these methods 3. Clinical features of CLL at demonstration are heterogeneous, and a de?nitive diagnosis is based on the combination of peripheral blood B-cell lymphocytosis (5109/L), characteristic morphology, and immunophenotype. Matutes ?ow cytometry score is particularly useful for differentiating between CLL and additional MBN4, 5 CLL classically offers score of 4 or 5 5. However, some CLL cases may have an atypical immunophenotype and/or morphological features leading to diagnostic confusion 6. Compact disc160 can be an Ig- like activating organic killer (NK) cell receptor7,8.The CD160 gene is on chromosome 1q42.39, CHMFL-ABL/KIT-155 expressed of all circulating NK cells and on a subset of circulating cytotoxic T cells, however, not on B cells 7,8. Binding of Compact disc160 to both traditional and non-classical main histocompatibility complicated course I enhances Compact disc8+cytotoxic-T and NK lymphocytes features10-13, aswell as cytokine creation, including IFN-? TNF-, and IL611,12. Latest work has showed Compact disc160 appearance in malignant B cells 14. Compact disc200, is a sort I glycoprotein that’s portrayed on thymocytes, turned on T cells, B cells, dendritic cells, endothelial cells, and neurons however, not on NK cells15,16. The Compact disc200 gene CHMFL-ABL/KIT-155 is normally mapped Lepr to chromosome 3q13.217,18. Compact disc200 interacts using the Compact disc200 receptor, which CHMFL-ABL/KIT-155 is normally restricted to antigen delivering cells of myeloid origins and a subset of T cells15, leading to immunosuppressive features19. Compact disc200 appearance was reported in CLL versus detrimental appearance in MCL. The appearance of Compact disc200 was reported in HCL, multiple myeloma, lymphoblastic lymphoma/leukemia, lymphoplasmacytic lymphoma, severe myeloid leukemias, and additional non-hematologic malignancies20. Lately, it’s been reported that CDs160/200 had been expressed generally of CLL21,22. In the lack of histological and/or cytogenetics/molecular extra explorations, the addition of CDs160/200 manifestation recognition in atypical B-CLPD proliferative syndromes of uncertain analysis could help to attain a de?nitive conclusion and better orientate individuals toward the most likely therapy. Goal of the task: Our function aimed to research the manifestation of CDs160/200 in CLL and additional MBN individuals and their make use of as yet another diagnostic device for differentiating CLL from additional MBN. Components AND Strategies Our research was completed on 60 diagnosed adult individuals with CLPDs newly; based on the immunophenotypic evaluation, individuals had further analysis as 30 individuals with CLL, 25 individuals with B-NHL (4 mantle cell lymphoma (MCL) and 2 follicular lymphoma (FL) instances had been one of them group) and 5 individuals with HCL, who have been recruited through the hematology device in Primary Alexandria University medical center through the period from November 2016 to Oct 2017. The analysis of CLL was predicated on the WHO 2016 diagnostic requirements according to regular requirements of microscopic cell morphology and ?ow cytometry evaluation. MCL.