Data CitationsChan L. KC, Seizl M, Tresch A, Cramer P. 2012.

Data CitationsChan L. KC, Seizl M, Tresch A, Cramer P. 2012. Cramer (2) mRNA halflife. Electron Microscopy Data Bank. E-MTAB-760Wang Y, Liu CL, Storey JD, Tibshirani RJ, Herschlag D, Brown PO. 2002. Brown (1) and (2) mRNA halflife. Stanford Genomic Resources. Yuleidatafiles/halflifeTable.txtDuttagupta R, Tian B, Wilusz CJ, Khounh DT, Soteropoulos P, Ouyang M, Dougherty JP, Peltz SW. MS-275 inhibitor database 2005. Peltz mRNA halflife, Supplementary Tables S1, S2, and S3. PubMed Central. molcellb_25_13_5499__index.htmlPresnyak V, Alhusaini N, Chen YH, Martin S, Morris N, Kline N, Olson S, Weinberg D, Baker KE, Graveley BR, Coller J. 2015. Coller (1) and (2) mRNA halflife, Table S1. PubMed Central. NIHMS665436-supplement-5.xlsGrigull J, Mnaimneh WBP4 S, Pootoolal J, Robinson MD, Hughes TR. 2004. Hughes mRNA stability. Hughes Lab, University of Toronto. Grigull/Fig2A_data.xlsHolstege FC, Jennings EG, Wyrick JJ, Lee TI, Hengartner CJ, Green MR, Golub TR, Lander ES, Young RA. 1998. Young mRNA stability. Young Lab, Whitehead Institute for Biomedical Research. young/expression.htmlGeisberg JV. 2014. Struhl mRNA stability, Table S2. PubMed Central. NIHMS552811-supplement-2.xlsxShalem O, Dahan O, Levo M, Martinez MR, Furman I, Segal E, Pilpel Y. 2008. Pipel mRNA stability, Supplementary Table 1. PubMed Central. msb200859-s2.xlsMunchel SE, Shultzaberger RK, Takizawa N. 2011. Weis (1) mRNA stability, Supplemental Data. Molecular Biology of the Cell. mc-e11-01-0028-s10.xlsPelechano V, Prez-Ortn JE. 2010. Perez-Ortin mRNA stability. Wiley Online Library. yea_1768_supportinforTS1.xlsSupplementary MaterialsFigure 1source data 1: Source data for Figure 1B: decay kinetics of ACT1, CIS3 and RPL25 mRNAs. elife-32536-fig1-data1.txt (220K) DOI:?10.7554/eLife.32536.005 Figure 1source data 2: Source data for Figure 1C and Figure 1figure supplement 1C: transcriptome wide decay data for two biological replicates of wild-type yeast cells grown in exponential phase. elife-32536-fig1-data2.txt (220K) DOI:?10.7554/eLife.32536.006 Figure 1figure supplement 2source data 1: Source MS-275 inhibitor database data for Shape 1figure health supplement 2ACB: transcriptome wide decay data ready without polyA selection. elife-32536-fig1-figsupp2-data1.txt (121K) DOI:?10.7554/eLife.32536.007 Figure 3source data 1: Resource data for Figure 3B, C, DCG, I and J: decay kinetics of selected mRNAs in cells treated with translational inhibitors. elife-32536-fig3-data1.xlsx (53K) DOI:?10.7554/eLife.32536.014 Figure 3source data 2: Resource data for Figure 3H: Transcriptome wide decay data for cells treated or mock treated with 3AT. elife-32536-fig3-data2.txt (132K) DOI:?10.7554/eLife.32536.015 Figure 3source data 3: Resource data for Figure 3K: Transcriptome wide decay data for cells treated with DMSO, hippuristanol or cycloheximide. elife-32536-fig3-data3.txt (162K) DOI:?10.7554/eLife.32536.016 Shape 4source data 1: Resource data for Shape 4B, C and F: accumulation kinetics of decay and P-bodies of RNA in P-bodies in cells treated with translational inhibitors. elife-32536-fig4-data1.xlsx (44K) DOI:?10.7554/eLife.32536.021 Supplementary file 1: Resources of MS-275 inhibitor database data for Shape 2. elife-32536-supp1.docx (16K) DOI:?10.7554/eLife.32536.022 Supplementary document 2: Candida strains. elife-32536-supp2.docx (17K) DOI:?10.7554/eLife.32536.023 Supplementary file 3: Plasmids. elife-32536-supp3.docx (15K) DOI:?10.7554/eLife.32536.024 Supplementary file 4: qPCR primers. elife-32536-supp4.docx (14K) DOI:?10.7554/eLife.32536.025 Supplementary file 5: Plasmid sequences. elife-32536-supp5.fa (176K) DOI:?10.7554/eLife.32536.026 Transparent reporting form. elife-32536-transrepform.pdf (319K) DOI:?10.7554/eLife.32536.027 Data Availability StatementSequencing data have already been deposited in GEO under accession code “type”:”entrez-geo”,”attrs”:”text message”:”GSE119560″,”term_identification”:”119560″GSE119560. The next dataset was generated: Chan L. 2018. mRNA balance mainly because measured by thiouracil incorporation in the absence and existence of translational inhibitors. NCBI Gene Manifestation Omnibus. GSE119560 The next previously released datasets were utilized: Nagalakshmi U, Wang Z, Waern K, Shou C, Raha D. 2008. mRNA great quantity and transcript limitations. PubMed Central. NIHMS229938-supplement-supp__dining tables.xls Subtelny AO, Eichhorn SW. 2014. polyA tail size. NCBI Gene Manifestation Omnibus. GSE52809 Brar GA, Yassour M, Friedman N, Regev A, Ingolia NT, Weissman JS. 2012. translational effectiveness. NCBI Gene Manifestation Omnibus. GSE34082 Drummond DA, Raval A, Wilke CO. 2006. CAI. Molecular Evolution and Biology. en-cai-fop.txt Pechmann S, Frydman J. 2013. nTE. Frydman Laboratory. codons/normalizedTE.txt Neymotin B, Athanasiadou R, Gresham D. 2014. Gresham mRNA halflife, Supplemental desk 5. PubMed Central. supp_20_10_1645__index.miller C html, Schwalb B, Maier K, Schulz D. 2011. Cramer (1) mRNA halflife. PubMed Central. msb2010112-s1.txt Sunlight M, Schwalb.

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